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KMID : 0366119750030010007
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Korean Journal of Applied Microbiology & Bioengineering
1975 Volume.3 No. 1 p.7 ~ p.15
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Studies on the L-Glutamic acid Fermentation(Part Ⅰ)
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梁漢喆/Yang, Han-Chul
崔瑢鎭/梁漢宇/成河珍/Choi, Yong-Jin/Yang, Han Woo/Sung, Ha-Chin
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Abstract
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The possibilities of utilizing acid-hydrolyzate of "Sliced and dried sweet potatoes" as a carbon source for the microbial production of L-Glutamic Acid(L-GA) with Micrococcus glutamicus were investigated and the results showed as follows:
1) The highest hydrolysis rate, 74.6% of the reducing sugar based on the weight of dry matter, was obtained when the sweet potatoes were hydrolyzed with 0.8% of HCl at 2.0kg/㎠ for 30 minutes.
The most favorable hydrolyzate for the growth of the cells, however, was found to be the one obtained by treating the sweet potatoes with 0.5% HC1 at 2.0kg/㎠ for 10 minutes. educing sugar content of the hydrolyzate was 10 % as glucose.
2) Biotin content of the hydrolyzate was 25μg/l and it was proved to be excess in amount or the L-GA production.
3) The effects of addition of antibiotics, alcohols and fatty acid esters on the L-GA production were tested in the biotin excess medium.
The production of L-GA was most increased to 32. 5g/l with the addition of 10 I. U. of penicillin per ml to the culture medium at 4 hours after inoculation.
But the addition of alcohols, especially fatty acid esters, showed no significant effects.
4) Among the organic nutrients tested," Gluten acid- hydrolyzate" greatly enhanced the production of L-GA adding it´s concentration of 1.0% to the medium.
5) The maximum production of L-GA resulted in 35g/l when the cells were grown for 48 hours in the hydrolyzate medium supplemented with 1. 0% of "Gluten acid hydrolyzate" and with 10 I. U. of penicillin per ml added at 4 hours after cultivation.
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